aprE [2017-8-11 16:0:3]

major extracellular alkaline protease

Locus: BSU10300

Isoelectric point: 9.34

Molecular weight: 39.37 kDa

Protein length: 381 aa

Gene length: 1143 bp

Function: protein degradation

Product: extracellular alkaline serine protease (subtilisin E))

Essential: no

Synonyms: sprE

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of proteins
• Information processing → Protein synthesis, modification and degradation → Proteolysis → Extracellular feeding proteases
• Groups of genes → Secreted proteins

Gene

Coordinates: 1,104,423 → 1,105,568

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)

Protein family

• peptidase S8 family (according to Swiss-Prot)

Structure

• 1SBC

Localization

• secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: aprE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • SinR: repression, PubMed, in SinR regulon
  • ScoC: repression, PubMed, in ScoC regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed by SinR PubMed
  • induced in the presence of external ComX PubMed

  Additional information

  • in a triple abrB codY scoC mutant, expression occurs during exponential growth PubMed

Biological materials

Mutant

• BKE10300 (ΔaprE::erm trpC2), available at BGSC PubMed
• KO7 (ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr), available as BGSC 1A1133

Antibody

• **

References

Reviews

Loading

Original publications

Loading